Student Seminar
Name: Mr. Kunnam Teja Vardhana Reddy
Title: Super resolution microscopy – “Imaging at Nanometric resolution”
Date & Time: Thursday, 10th October 2024 at 4.00 p.m.
Venue: Rajarshi Bhattacharya Memorial Lecture Hall, Chemical Sciences Building
Abstract:
In modern scientific research, various types of microscopes are employed to visualize specimens at different resolutions. The resolution of a visible light microscope is commonly taken to be about 250 nm. Any sparse objects smaller than this dimension, will appear under the microscope as diffraction-limited spots. Super-resolution microscopy represents a ground breaking advancement in imaging technology, allowing researchers to observe structures and processes at a resolution beyond the diffraction limit of conventional light microscopy. The Nobel Prize in Chemistry 2014 was awarded jointly to Eric Betzig, Stefan W. Hell and William E. Moerner “for the development of super-resolved fluorescence microscopy”. In this presentation, the fundamental limits of optical resolution based on Abbe’s diffraction limit and Rayleigh’s criterion, and the evolution of microscopy over the past two centuries, focusing on advancements in resolution using visible light will be discussed. Some of the super resolution techniques viz. structured illumination microscopy (SIM), stimulated emission depletion microscopy (STED) and single molecule localization microscopy (SMLM) highlighting their principle and the improvement in the resolution up to nanometer scale will be discussed. I will discuss the application of super resolution microscopy in probing monomer exchange mechanism between supramolecular polymers and observing catalysis of a single nanoparticle.
References:
1. Albertazzi et.al., Super-Resolution Microscopy as a Powerful Tool to Study Complex Synthetic Materials. Nat. Rev. Chem.2019, 3 (2), 68–84
2. Zhou et.al., Scalable Parallel Screening of Catalyst Activity at the Single-Particle Level and Subdiffraction Resolution. ACS Catal.2013, 3 (7), 1448–1453
3. van de Linde et.al., Investigating Cellular Structures at the Nanoscale with Organic Fluorophores. Chem. Biol. 2013, 20 (1), 8–18
4. Thompson et.al., Precise Nanometer Localization Analysis for Individual Fluorescent Probes. Biophys. J. 2002, 82 (5), 2775–2783
5. Albertazzi et.al., Probing Exchange Pathways in One-Dimensional Aggregates with Super-Resolution Microscopy. Science 2014, 344 (6183), 491–495