Speaker: Asha Sweety Thomas
Topic: Super Resolution Fluorescence Microscopy
Date & Time: Thursday, 11th February 2021 at 4:00 PM through MICROSOFT TEAMS
Link to Microsoft Teams platform:
Abstract:
The fluorescence microscope is a powerful and non-invasive imaging tool owing to its ability to image live samples labelled with specific fluorescent markers. However, the resolution of a conventional microscope is diffraction limited which makes it difficult to resolve objects closer than few hundreds of nanometers. The non-invasive nature of the light microscope makes it highly desirable to improve the resolution beyond this limitation to image the sub cellular components and associated biochemical processes. Super-resolution fluorescence microscopic techniques are a set of techniques which have been introduced to circumvent the diffraction limit of light microscope and enhance the resolution of microscopic imaging down to a few nanometers.
The different super resolution techniques that are being currently used can be conceptually classified as the derivatives of the three main super resolution techniques – SIM, STED and PALM – which will be discussed in the talk. I will be discussing the conceptual basis which makes these techniques possible, as well as the new science enabled by super-resolution microscopy.
References:
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- Wu, Y.; Shroff, H., Nature methods 2018,15 (12), 1011-1019.
- Gustafsson, M. G. Proceedings of the National Academy of Sciences 2005,102 (37), 13081-13086.
- Hell, S. W.; Sahl, S. J.; Bates, M.; Zhuang, X.; Heintzmann, R.; Booth, M. J.; Bewersdorf, J.; Shtengel, G.; Hess, H.; Tinnefeld, P., Journal of Physics D: Applied Physics 2015, 48 (44), 443001.