Student Seminar
Name: Ms. Parineeta Gogoi
Title- Microsphere Nanoscopy: Super-resolution by dielectric microlenses
Date & Time: Thursday, 21st March at 4.00 p.m.
Venue: Rajarshi Bhattacharya Memorial Lecture Hall, Chemical Sciences Building
Abstract:
Diffraction limits the image resolution of a conventional optical microscope to ~200 nm in the visible spectrum.Another factor limiting resolution is the loss of evanescent waves in the far field. To address these constraints, researchers have developed optical nanoscopes. One such technique is Microsphere Nanoscopy, introduced by Wang et al. in 2011. This label-free, real-time imaging technique achieves a remarkable 50-nm-resolution by utilizing transparent dielectric microspheres as super-resolution lenses, effectively overcoming the diffraction limit of white light.
In my seminar, I will talk about the fundamentals of how optical diffraction and evanescent wave loss in the far field limit resolution. Then, I will talk about the mechanisms used to achieve super-resolution imaging, focusing on two techniques: the Photonic Nanojet (PNJ) and the Super-Resonance Effect (SRE), a new form of Whispering Gallery Mode (WGM). I will conclude by discussing applications for super resolution imaging focusing on its utilization in visualizing adenoviruses through submerged microsphere nanoscopy.
References:
Wang, Z., Guo, W., Li, L. et al. Optical virtual imaging at 50 nm lateral resolution with a white-light nanoscope. Nat Commun 2, 218 (2011).
Li, L., Guo, W., Yan, Y. et al. Label-free super-resolution imaging of adenoviruses by submerged microsphere optical nanoscopy. Light Sci Appl 2, e104 (2013).
Astratov, Vasily, ed. Label-free super-resolution microscopy. Cham: Springer, (2019).
Boris S. Luk’yanchuk, Ramón Paniagua-Domínguez, Igor Minin, Oleg Minin, and Zengbo Wang. Refractive index less than two: photonic nanojets yesterday, today and tomorrow. Opt. Mater. Express 7, 1820-1847 (2017).